Table 6. T_m-values and thermodynamic data of non self-complementary ps duplexes of the sequence 5′-d(TiCATAAiCTXXAT)·5′-d(AGTATTGAYCTA)^a,b.

X·Y		Tm (°C)	ΔTm/mod (°C)	X·Y		Tm (°C)	ΔTm/mod (°C)	X·Y		Tm (°C)	ΔTm/mod (°C)
iG·Cc	23·24	44
4·G	25·35	32	–12	5·G	26·35	38	–6	6·G	27·35	39	–5
4·T	25·36	29	–15	5·T	26·36	34	–10	6·T	27·36	34	–10
4·A	25·37	30	–14	5·A	26·37	33	–11	6·A	27·37	32	–12
4·iC	25·38	40	–4	5·iC	26·38	45	+1	6·iC	27·38	46	+2

^aIn 1 M NaCl, 0.1 M MgCl₂, 60 mM sodium cacodylate, pH 7; oligonucleotide concentration: 5 µM (single strand).

^bd(iC) = ^MeiC_d = 5-Methyl-2′-deoxyisocytidine.

^cSee Seela and Wei (22).