Table 7. Tm-values and thermodynamic data of non self-complementary aps duplexesa,b.
| Tm (°C) | ΔTm/mod (°C) | ΔH° (kcal/mol) | ΔS° [cal/(mol K)] | ΔG°310 (kcal/mol) | ||
|---|---|---|---|---|---|---|
| 3′-d(ATCCAGTTATGA) | 24 | 51 | –85 | –237 | –11.6 | |
| 5′-d(TAGGTCAATACT) | 29 | |||||
| 3′-d(TAGGTCAATACT) | 29a | 49 | –97 | –275 | –11.5 | |
| 5′-d(ATCCAGTTATGA) | 24a | |||||
| 3′-d(TAiG iGTiCAATAiCT)c | 23 | 60 | –94 | –257 | –14.8 | |
| 5′-d(AT iC iCAiG TTATiGA) | 28 | |||||
| 3′-d(TA 4 4T iCAATA iCT) | 25 | 61 | +0.25 | –96 | –263 | –14.6 |
| 5′-d(ATiCiCA 4 TTAT 4 A) | 30 | |||||
| 3′-d(TA 5 5 T iCAATAiCT) | 26 | 69 | +2.25 | –106 | –283 | –17.6 |
| 5′-d(ATiCiCA 5 TTAT 5 A) | 35 | |||||
| 3′-d(TA 6 6 T iCAATAiCT) | 27 | 73 | +3.25 | –119 | –321 | –20.0 |
| 5′-d(ATiCiCA 6 TTAT 6 A) | 36 |
aMeasured in 1 M NaCl, 0.1 M MgCl2, 60 mM sodium cacodylate, pH 7; oligonucleotide concentration: 5 µM (single strand).
bd(iC) = MeiCd = 5-Methyl-2′-deoxyisocytidine.
cSee Seela et al. (4).