Table 8. T_m-values and thermodynamic data of non self-complementary aps duplexes of the sequence 5′-d(TAGGTXAATACT)·3′-d(ATCCAYTTATGA)^a,b.

X·Y		Tm (°C)	ΔTm/mod (°C)	X·Y		Tm (°C)	ΔTm/mod (°C)	X·Y		Tm (°C)	ΔTm/mod (°C)
iC·iGc	34·39	54
iC·4	34·40	56	+2	iC·5	34·41	57	+3	iC·6	34·42	58	+4
C·4	29·40	42	–12	C·5	29·41	47	–7	C·6	29·42	48	–6
G·4	31·40	42	–12	G·5	31·41	47	–7	G·6	31·42	47	–7
T·4	32·40	41	–13	T·5	32·41	42	–12	T·6	32·42	42	–12
A·4	33·40	28	–26	A·5	33·41	27	–27	A·6	33·42	28	–26

^aIn 1 M NaCl, 0.1 M MgCl₂, 60 mM sodium cacodylate, pH 7; oligonucleotide concentration: 5 µM (single strand).

^bd(iC) = ^MeiC_d = 5-Methyl-2′-deoxyisocytidine.

^cSee Seela and Wei (22).