Skip to main content
. 2003 Dec 15;31(24):7227–7237. doi: 10.1093/nar/gkg937

Figure 2.

Figure 2

Nuclease-dependent cell cycle delay induced by SCPVU-TAT. Cell cycle distribution of protein-transduced U2OS cells by FACS. 2n presents relative diploid, not replicated equivalent of DNA (G1), 4n presents relative tetraploid, replicated equivalent of DNA (G2/M). Ploidy indications are valid for both histogram rows. U2OS cells were grown for 24 h (upper row) or 48 h (lower row) in the presence of increasing concentrations of SCPVU-TAT (10, 50, 100 nM) as indicated and with one single addition of enzyme, or with multiple additions of enzymes (every 12 h, 10 nM*12, 100 nM*12); no treatment (control); nuclease-deficient version of SCPVU-TAT (SC34, 100 nM). All histogram plots that are shown represent a measure of the DNA content versus cell numbers as indicated only for the upper left histogram. The percentage of cells in the various cell cycle phases is given below each histogram.