Figure 1.

Multiple pseudopodia formation induced by nadPH inhibitor diphenyleneiodonium chloride (dPI) was more prominent in relatively shallow chemoattractant gradient. (a) relatively shallow chemoattractant gradient was generated in the upper section in the eZ-taxiscan device. (B) dependent on rOS in neutrophil chemotaxis relies on the feature of the gradient. neutrophils were treated with 50 µM dPI for 30 min and chemotaxis was induced by 100 nM fMLP. neutrophil purification, eZ-taxiscan chemotaxis assay, and analysis of cell tracks and morphology were conducted as previously described.14,18,19 Percentage of cells that display multiple pseudopodia (n = 20 cells, Fisher’s 2 × 2 test, *p < 0.05 versus untreated) during the course of the eZ-taxiscan chemotaxis assay was quantified as described by Hattori et al.14