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. 2003 Dec 15;31(24):7208–7215. doi: 10.1093/nar/gkg945

Figure 5.

Figure 5

Interference of antibiotics with the chloramphenicol-dependent modifications. Complexes were formed between ribosomes (0.15 µM) and chloramphenicol (1.2 mM) by incubating at 37°C for 10 min. A second antibiotic was added and incubation was continued at 37°C for another 20 min. The samples were irradiated for 30 min at 365 nm, followed by isolation of rRNA. Primer extension analysis was carried out using primers (A) Ec2654 for the modifications at C2611/C2612 in E.coli and (B) Hh2133 for the modification at G2084 (2058) in H.halobium. The drugs used were anisomycin (Ani), blasticidin S (Bla), carbomycin (Car), celesticetin (Cel), erthyromycin (Ery), gougerotin (Gou), lincomycin (Lin), pristinamycin IIA (PIIA) and puromycin (Pur). All antibiotics were used at a concentration of 200 µM with the exception of puromycin, which was used at a concentration of 1 mM. The lanes labeled G, A, U and C represent sequencing tracks.