Figure 3.

Hydrolysis of the N-glycosidic bond at 37°C. 1-Deaza-dG (1) at pH 2.2 (closed circles), pH 3.1 (closed triangles), pH 4.1 (closed squares) and pH 5.4 (closed diamonds). Deoxyguanosine at pH 2.2 (open circles) and 4.1 (open squares). Conditions used are described in Materials and Methods. Deglycosylation was monitored by RP-HPLC and the remaining percentages were calculated by comparing the ratio of compounds with thymine.