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Single nucleotide insertion studies with Klenow fragment (exo–). (A) Sequences of a 25 nt template (III) and 20 nt primer (IV). (B) PAGE of dNTP incorporation opposite the 1-deaza-dG residue. (C) PAGE of 1-deaza-dGTP incorporation opposite a natural nucleoside residue. Conditions used are described in Materials and Methods. Both X in the template sequence and added dNTP are indicated at the top of the gel.
