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. 2003 Dec 15;31(24):7175–7188. doi: 10.1093/nar/gkh154

Figure 5.

Figure 5

PAGE of single nucleotide insertion studies (A) with Therminator™ DNA polymerase (reaction mixtures being incubated at 74°C for 10 min), (B) with AMV reverse transcriptase (reaction mixtures being incubated at 37°C for 30 min) and (C) with M-MLV reverse transcriptase (reactions mixture being incubated at 37°C for 30 min). Conditions used are described in Materials and Methods. Both X in the template sequence and added dNTP are indicated at the top of each gel. Z indicates 1-deaza-dGTP; P indicates reaction without dNTP.