. Author manuscript; available in PMC: 2010 Aug 10.

Published in final edited form as: Cell Mol Bioeng. 2009 Sep 1;2(3):386–394. doi: 10.1007/s12195-009-0070-x

TABLE 2.

Average staining intensity for type II collagen, type IX collagen, and COMP immunohistochemistry for constructs cultured for 28 days in free swelling (FS) conditions or under dynamic loading for 1 h on/off (DL 1 h on/off), 3 h continuously (DL 3 h), and 6 h continuously (DL 6 h).

Day 28 immunohistochemistry average staining intensity

	FS		DL 1 h on/off		DL 3 h		DL 6 h

	Edge	Center	Edge	Center	Edge	Center	Edge	Center
Type II collagen	43.000 ± 5.196	42.197 ± 4.477	41.557 ± 4.822	42.103 ± 4.490	83.948 ± 10.074^†	70.907 ± 9.411^†	142.611 ± 18.568^*^†	102.001 ± 12.061^†
Type IX collagen	29.342 ± 4.192	27.678 ± 3.202	29.582 ± 3.356	27.647 ± 3.186	29.479 ± 3.741	30.641 ± 4.084	40.675 ± 5.581^†	35.998 ± 3.936^†
COMP	81.825 ± 11.591	54.360 ± 7.412	83.914 ± 9.060	56.366 ± 6.448	96.823 ± 14.080	74.061 ± 10.075	109.150 ± 12.917^*,^†	64.920 ± 8.736

p < 0.05 vs. center,

^†

p < 0.05 vs. FS of respective location.