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. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: Chromatographia. 2010 Jan 1;71(1-2):37–53. doi: 10.1365/s10337-009-1409-0

Fig. 12.

Fig. 12

Fluorescence spectra of ABS-tagged 5-hydroxytryptophan (a), the dependence of the fluorescence intensity (excitation and emission wavelengths of 360 and 490 nm, respectively) on 5-OH-Trp concentration (b), and fluorescence resulted from the incubation for 1 h of 50 μM Trp with 2 mM ABS and 10 μM K3Fe(CN)6) in 0.1 M PBS (pH 9) at room temperature (c). The excitation spectrum for 1 μM 5-OH-Trp (the emission wavelength was set at 500 nm) is shown in panel a (dashed line)