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. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: Chromatographia. 2010 Jan 1;71(1-2):37–53. doi: 10.1365/s10337-009-1409-0

Fig. 15.

Fig. 15

Fluorescence imaging of ABS-tagged proteins after SDS-PAGE separation Line 1: molecular weight standard (Precision Plus Dual Color, Bio-Rad); line 2: 5 μg rabbit phosphorylase b (containing 50 pmol 3-NT), non-derivatized; line 3: same protein, exposed to ABS without 3-NT reduction; line 4: same protein, 3-NT reduced and ABS-tagged; line 5: lysate of C2C12 cells exposed to peroxynitrite (50 μg protein containing 500 pmol 3-NT), after 3-NT reduction and ABS-tagging; line 6: same preparation, non-reduced, ABS tagged; line 7: same preparation, non-reduced and non-tagged control