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. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: Chromatographia. 2010 Jan 1;71(1-2):37–53. doi: 10.1365/s10337-009-1409-0

Fig. 16.

Fig. 16

Representative tandem MS spectra of ABS tagged nitrated C2C12 proteins obtained by nanoLC–FTICR–MS–MS analysis of in-gel digests. Reduction of 3-NT (with 10 mM Na2S2O4) and tagging with 2 mM ABS and 20 μM K3Fe(CN)6 in 100 mM Na2HPO4, pH 9.0, was performed on non-digested protein samples (containing 100 μg protein and 5 μM 3-NT in a 100-μL volume). MS–MS spectra show the type I product (Y + 366 AMU) of ABS tagged sequences for murine actin peptide GY*SFTTTAER (a), and murine lactate dehydrogenase peptide QVVDSAY*EVIK (b)