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. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: Chromatographia. 2010 Jan 1;71(1-2):37–53. doi: 10.1365/s10337-009-1409-0

Fig. 3.

Fig. 3

Fluorescence tagging of FSAY(3-NH2)LER analyzed by RP–LC with UV detection at 214 nm (a) and by fluorescence detection with excitation and emission wavelengths at 360 and 490 nm, respectively (b). Chromatograms 1 and 2 are recorded before and after tagging of 50 μM FSAY(3-NH2)LER with 10 mM ABS and 0.5 mM K3Fe(CN)6 for 1 h at room temperature. The UV spectra of major peaks analyzed by a PDA detector are shown in the insert to a; the fluorescence spectrum of a total sample is shown in the insert to panel b