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. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: Chromatographia. 2010 Jan 1;71(1-2):37–53. doi: 10.1365/s10337-009-1409-0

Fig. 7.

Fig. 7

Determination of the fluorescence quantum yield for RP–LC-purified FSAY(A2)LER (peak at ca. 16 min in the panel a) using a reference fluorescence peptide, the GSH-ThioGlo1 adduct. Panel b shows absorbance (Abs.), fluorescence excitation (Ex.) and emission (Em.) spectra for 1 μM FSAY(A2)LER (solid lines) and 2 μM ThioGlo1-GSH (dashed lines) in 0.1 M NaH2PO4, pH 7.4