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. 2010 Sep;51(9):2629–2641. doi: 10.1194/jlr.M005132

Fig. 2.

Fig. 2.

A: Immunoblot with mAb 210 (monoclonal antibody against the α subunit of the T. californica AChR) of DRM (P) and DSM (S) fractions obtained from purified AChR reconstituted in POPC:SM:Chol containing GM1 (i) without further treatment, (ii) treated with cholera toxin B subunit (CTxB) for GM1 crosslinking, and (iii) treated with CTxB for GM1 crosslinking followed by anti-cholera toxin antibody (anti-CTx) for crosslinking of CTxB. B: Immunoblot of DSM (S) and DRM (P) fractions obtained from purified AChR reconstituted in a POPC:SM:Chol 1:1:1 system in the presence of a rapsyn-enriched extract in three different rapsyn:AChR molar stoichiometries (1:1, 2:1, and 4:1). Blots were probed with mAb 1234 (monoclonal antibody against rapsyn) and mAb 210 (monoclonal antibody against the α subunit of the T. californica AChR). Panels A and B are representative of at least three independent experiments. AChR, nicotinic acetylcholine receptor; Chol, cholesterol; CTx, cholera toxin; CTxB, cholera toxin B-subunit; DRM, detergent-resistant membrane; DSM, detergent-soluble membrane; GM1, ganglioside GM1; POPC, palmitoyloleylphosphatydilcholine; SM, brain sphingomyelin.