Gibbs triangle showing DRM (solid) and DSM (open) compositions for all the
systems tested in this work: DRM-like (▪), DSM-like (□),
different raft-containing membranes (i) in the absence of
AChR (•, ○), (ii) in the presence of γM4 peptide (1:250
▴, △), and (iii) in the presence of different AChR:lipid molar
ratios (1:400, ▾, ▽; and 1:130, ♦, ⋄). The DRM and
DSM lipid compositions proposed in the work of Schroeder et al. (50) (◂, ◃) and McIntosh et
al. (51) (▸, ▹) are
indicated. Also shown are the lines that divide the phase diagram in the
different pure phases (solid, s; liquid-ordered,
lo; and liquid disordered,
ld) and the different coexistence
regions at 22°C (s + lo;
ld + lo + s;
ld + s; and ld
+ lo) proposed by Pokorny et al. (70). AChR, nicotinic acetylcholine
receptor; BSM, brain sphingomyelin; Chol, cholesterol; DRM,
detergent-resistant membrane; DSM, detergent-soluble membrane; POPC,
palmitoyloleylphosphatydilcholine.