Examples of tip-to-tip and tip-to-side vegetative hyphal fusions. (A) Series of images from Movie S5 in the supplemental material showing an example of tip-to-side fusion between a GFP-tagged hyphal compartment (black arrow) and a ChFP-tagged germinating conidium (white arrow). After fusion, the ChFP-tagged nucleus divides, and one daughter nucleus (white arrowheads) migrates through the fusion bridge into the neighboring cell, moving past a GFP-tagged nucleus (black arrowheads), whose fluorescence disappears after 25 min. (B) Series of images from Movies S6 and S7 in the supplemental material showing an example of tip-to-tip fusion. Two apical cells home toward each other (black arrowheads), fuse, and converge into a single hyphal compartment led by the mitotically active, GFP-tagged nucleus (white arrowheads). Note that after fusion, both a GFP- and a ChFP-tagged nucleus coexist inside the same cell for approximately 429 min, followed by degradation of the ChFP-tagged nucleus. s, septum.