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Strategy for targeted replacement of MET12 (A) and MET13 (B) in F. graminearum. (C) PCR-based analysis of transformants using four different primer pairs (listed above the lanes) for each of the two loci. Lanes L, 2 log ladder; lanes M, mutant; lanes W, wild type; lanes 0 water control. (D) Southern blot analysis of T-DNA copy number in the selected mutant indicated by laboratory designations using a 789-bp fragment of the hph gene as probe.
