Phylogeny made with the neighbor-joining algorithm, based on a global alignment (ClustalW) of the identified MTHFR amino acid sequences from the following fungi, bacteria (E. coli), metazoans, Viridiplantae, and Dictyosteliida (species abbreviations): Ajellomyces capsulatus NAm1 (Ajc), Ascosphaera apis USDA-ARSEF 7405 (Aa), Aspergillus clavatus NRRL 1 (Ac), Aspergillus flavus NRRL3357 (Af), Aspergillus fumigatus Af293 (Afu), Aspergillus nidulans FGSC A4 (An), Aspergillus oryzae RIB40 (Ao), Aspergillus terreus ATCC 20542 (At), Botryotinia fuckeliana B05.10 (Bf), Candida albicans SC5314 (Ca), Candida dubliniensis CD36 (Cd), Candida glabrata CBS138 (Cgl), Candida tropicalis MYA-3404 cont1.60 (Ct), Chaetomium globosum CBS 148.51 (Cg), Clavispora lusitaniae ATCC 42720 cont1.29 (Cl), Coccidioides immitis H538.4 (Ci), Debaryomyces hansenii CBS767 (Dh), Eremothecium gossypii (Eg), Gibberella moniliformis 7600 (GM), Gibberella zeae PH-1 (Fg), Kluyveromyces lactis NRRL Y-1140 (Kl), Kluyveromyces waltii NCYC 2644 cont306 (Kw), Lodderomyces elongisporus NRRL YB-4239 cont1.24 (Le), Magnaporthe grisea 70-15 (MG), Neosartorya fischeri NRRL 181 (Nf), Neurospora crassa (Nc), Phaeosphaeria nodorum SN15 (Pn), Saccharomyces bayanus MCYC 623 (Sb), Saccharomyces castellii NRRL Y-12630 (Sca), Saccharomyces cerevisiae (Sc), Saccharomyces kluyveri NRRL Y-12651 (SkN), Saccharomyces kudriavzevii IFO1802 (Sak), Saccharomyces mikatae IFO1815 (Sm), Saccharomyces paradoxus NRRL Y-17217 (Spa), Schizosaccharomyces pombe 972 h− (Sp), Sclerotinia sclerotiorum 1980 (Ss), Stagonospora nodorum SN15 (Sn), Trichoderma reesei QM9414 (Tr), Uncinocarpus reesii 1704 (Ur), Yarrowia lipolytica CLIB122 (Yl), Ustilago maydis 521 (Us), Coprinopsis cinerea okayama 7#130 (Cc), Cryptococcus neoformans var. neoformans B-3501A (Cn), Dictyostelium discoideum AX4 (Dd), Malassezia globosa CBS 7966 (Mag), Homo sapiens (Hs), Mus musculus (Mm), Bos taurus (Bt), Taeniopygia guttata (Tg), Danio rerio (Dr), Caenorhabditis elegans (Ce), Gallus gallus (Gg), Drosophila melanogaster (Dm), Anopheles gambiae strain PEST (Ag), Arabidopsis thaliana (Art), Physcomitrella patens subsp. patens (Pp), Oryza sativa (Os), and Chlamydomonas reinhardtii (Cr).