Phenotypes and chemotypes of F. graminearum wild-type, FgΔmet12, and FgΔmet13 strains. (A) Picture of wild-type and mutants grown for 5 days on DFM with variable l-methionine concentrations. (B to D) HPLC-DAD analysis at 280 nm of wild-type (B), FgΔmet12 (C), and FgΔmet13 (D) strains grown for 10 days on DFM with variable l-methionine concentrations (blue, 0 μg/ml; red, 20 μg/ml; green, 40 μg/ml; and pink, 60 μg/ml). (E to G) Recorded UV/visible light spectra for aurofusarin (E), rubrofusarin (F), and nor-rubrofusarin (G).