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. 2010 Jun 25;76(16):5500–5509. doi: 10.1128/AEM.00691-10

FIG. 6.

FIG. 6.

PCR amplification of apsA, qmoA, qmoC, and DVU0851 and the region spanning the junctions between apsA and qmoA and between qmoC and DVU0851 from D. vulgaris genomic DNA and cDNA. PCR amplification was performed on D. vulgaris genomic DNA (lanes 1 to 6) and cDNA from D. vulgaris grown in lactate-sulfate medium (lanes 8 to 13) for an internal fragment of apsA (expected size, 440 bp) (lanes 1 and 8), an internal fragment of qmoA (expected size, 610 bp) (lanes 2 and 9), a region spanning the intergenic region that includes coding sequences for the C terminus of ApsA and the N terminus of QmoA (expected size, 1,510 bp) (lanes 3 and 10), qmoC (expected size, 875 bp) (lanes 4 and 11), DVU0851 (expected size, 747 bp) (lanes 5 and 12), and a region spanning the intergenic region that includes coding sequences for the C terminus of QmoC and the N terminus of DVU0851 (expected size, 1,711 bp) (lanes 6 and 13). A 1-kb Plus DNA ladder (Fermentas) is in lanes 7 and 14. Sizes of observed bands are labeled on the left-hand side of the gel.