TABLE 2.
Dissolved sulfide generated by wild-type D. vulgaris, mutants, and complemented mutants when grown on lactate-sulfate or lactate-sulfite mediuma
| Strain (genotype) | Plasmid (complementing gene[s]) | Medium | Dissolved sulfide (mM) generated atb: |
|
|---|---|---|---|---|
| 0 h | 100 h | |||
| Wild type | No plasmid | Lac-SO4 | −0.4 ± 0.2 | 14.0 ± 2.5 |
| Lac-SO3 | −1.1 ± 1.5 | 24.7 ± 0.5 | ||
| JW9021 [Δ(qmoABC-DVU0851)] | No plasmid | Lac-SO4 | −0.3 ± 0.2 | −0.1 ± 0.2 |
| Lac-SO3 | −0.2 ± 0.2 | 20.9 ± 1.1 | ||
| pMO9116 (qmoABC) | Lac-SO4 | −0.4 ± 0.1 | 13.8 ± 1.2 | |
| Lac-SO3 | −0.3 ± 0.1 | 22.0 ± 0.2 | ||
| pMO9117 (qmoABC-DVU0851) | Lac-SO4 | −0.3 ± 0.1 | 10.4 ± 7.7 | |
| Lac-SO3 | −0.3 ± 0.1 | 23.6 ± 1.0 | ||
| pMO9118 (DVU0851) | Lac-SO4 | 0.0 ± 0.0 | 0.0 ± 0.1 | |
| Lac-SO3 | 0.0 ± 0.1 | 21.0 ± 3.7 | ||
| JW9063 (ΔDVU0851) | No plasmid | Lac-SO4 | 0.2 ± 0.1 | 14.7 ± 0.4 |
| Lac-SO3 | 0.5 ± 0.4 | 22.6 ± 0.8 | ||
| pMO9118 (DVU0851) | Lac-SO4 | 0.2 ± 0.1 | 13.6 ± 0.2 | |
| Lac-SO3 | 0.3 ± 0.1 | 23.1 ± 1.8 | ||
a
Lactate-sulfate, Lac-SO4 (60 mM-30 mM); lactate-sulfite, Lac-SO3 (60 mM-40 mM).
b
Values are averages of three biological replicates ± standard deviations.