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. 2010 Jun 25;76(16):5577–5584. doi: 10.1128/AEM.00648-10

FIG. 1.

FIG. 1.

Serotype 1/2a isolates A7 and SE076 with Sau3AI-resistant DNA harbor an RM cassette homologous to that in the ECI strain F2365 and in a genomically equivalent location. PCR primers used were as follows: lanes 1 to 4, strains F2365, A7, SE076, and EGD-e, respectively, with primers 0325F and 0327R, amplifying the putative restriction endonuclease gene and putative methyltransferase gene of F2365; lanes 5 to 8, strains F2365, A7, SE076, and EGD-e, respectively, with primers LMO0321RMdelF and LMO0325R amplifying a fragment spanning a portion of ORFs LMOf2365_0321 and LMOf2365_0325 in F2365; lanes 9 to 12, strains F2365, A7, SE076, and EGD-e, respectively, with primers LMO0328RMdelF and LMO0329RMdelR, amplifying a fragment spanning a portion of ORFs LMOf2365_0329 and LMOf2365_0328 in F2365; lanes 13 to 15, strains EGD-e, A35, and 10, respectively, with primers LMO0321RMdelF and LMO0329RMdelR, flanking the RM cassette of F2365; lane M, DNA size markers (exACTGene cloning DNA ladder; Fisher Scientific). Strains A35 and 10 had Sau3AI-resistant DNA but lacked an RM cassette in this genomic location. PCR was done as described in Materials and Methods and using the primers listed in Table 2.