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. 2010 Jun 25;76(16):5577–5584. doi: 10.1128/AEM.00648-10

FIG. 3.

FIG. 3.

Evidence for cytosine methylation at GATC sites in wild-type strains and mutants with deletion of the RM cassette. Lanes: 1 to 3, DNA from strain F2365, uncut, digested with Sau3AI, and digested with MboI, respectively; 4 and 5, DNA from deletion mutant DS86 uncut and digested with Sau3AI, respectively; 6 to 8, DNA from strain A7, uncut, digested with Sau3AI, and digested with MboI, respectively; 9 and 10, DNA from deletion mutant DS14 uncut and digested with Sau3AI, respectively; 11 to 13, DNA from FSL N3-165, uncut, digested with Sau3AI, and digested with MboI, respectively; M, molecular weight marker II (Roche Diagnostics, Indianapolis, IN). DNA digestions were as described in Materials and Methods.