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. 2010 Jun 25;76(16):5577–5584. doi: 10.1128/AEM.00648-10

TABLE 1.

L. monocytogenes strains used in this study

Strain ID Yr isolated Origin (reference) Serotype Sau3AI digestion MboI digestion RM probe reactivity
F2365 1985 California outbreak (25) 4b + +
DS86 This study; RM deletion mutant of strain F2365 4b + +
A7 1999 Guacamole 1/2a + +
DS14 This study; RM deletion mutant of strain A7 1/2a + +
A8 1999 Avocado pulp, Mexico 1/2a + +
A11 2001 Avocado pulp, Mexico 1/2a + +
A29 2002 Avocado pulp, Mexico 1/2a + +
A33 2003 Avocado pulp, Mexico 1/2a + +
A36 2003 Guacamole 1/2a + +
SE076 1986 Crab meat, frozen 1/2a + +
A95 2004 Avocado pulp 1/2a + +
FSL N3-165 2002 Soil, farm, New York (26a) 1/2a + NTb
FSL J1-208 1998 Goat, encephalitis (38a) 4a + NTb
A35a 2003 Guacamole 1/2a +
1a (255)a Unknown Lab QCc strain, North Carolina Dept. of Health 1/2a +
10a 2005 Environment, turkey processing plant (23) 1/2a +
2003-184Ra 2003 Human case, North Carolina 1/2a +
A22 2001 Sliced turkey 1/2a + +
A34 2003 Cuttlefish 1/2a + +
A38 2003 Frozen snow crab clusters 1/2a + +
A39 2003 Cooked baby clam meat 1/2a + +
A42 2001 Mozzarella curd 1/2a + +
A67 2001 Frozen capelin roe 1/2a + +
A74 2003 Avocado puree for ice cream 1/2a + +
A75 2003 Smoked salmon 1/2a + +
A94 2004 Cheese chile relleno 1/2a + +
A96 2004 Breaded catfish 1/2a + +
A97 2004 Avocado puree 1/2a + +
AT-05 1987 Raw milk 1/2a + +
AT-01 1986 Brie cheese 1/2a + +
a

These isolates were resistant to Sau3AI, but their RM cassettes were not homologous to those of strain F2365; the isolates are designated “x-RM” in Fig. 4.

b

NT, not tested. Genome sequence analysis revealed the presence of the RM genes, as described in Results.

c

QC, quality control.