TABLE 1.
Strain ID | Yr isolated | Origin (reference) | Serotype | Sau3AI digestion | MboI digestion | RM probe reactivity |
---|---|---|---|---|---|---|
F2365 | 1985 | California outbreak (25) | 4b | − | + | + |
DS86 | This study; RM deletion mutant of strain F2365 | 4b | + | + | − | |
A7 | 1999 | Guacamole | 1/2a | − | + | + |
DS14 | This study; RM deletion mutant of strain A7 | 1/2a | + | + | − | |
A8 | 1999 | Avocado pulp, Mexico | 1/2a | − | + | + |
A11 | 2001 | Avocado pulp, Mexico | 1/2a | − | + | + |
A29 | 2002 | Avocado pulp, Mexico | 1/2a | − | + | + |
A33 | 2003 | Avocado pulp, Mexico | 1/2a | − | + | + |
A36 | 2003 | Guacamole | 1/2a | − | + | + |
SE076 | 1986 | Crab meat, frozen | 1/2a | − | + | + |
A95 | 2004 | Avocado pulp | 1/2a | − | + | + |
FSL N3-165 | 2002 | Soil, farm, New York (26a) | 1/2a | − | + | NTb |
FSL J1-208 | 1998 | Goat, encephalitis (38a) | 4a | − | + | NTb |
A35a | 2003 | Guacamole | 1/2a | − | + | − |
1a (255)a | Unknown | Lab QCc strain, North Carolina Dept. of Health | 1/2a | − | + | − |
10a | 2005 | Environment, turkey processing plant (23) | 1/2a | − | + | − |
2003-184Ra | 2003 | Human case, North Carolina | 1/2a | − | + | − |
A22 | 2001 | Sliced turkey | 1/2a | + | + | − |
A34 | 2003 | Cuttlefish | 1/2a | + | + | − |
A38 | 2003 | Frozen snow crab clusters | 1/2a | + | + | − |
A39 | 2003 | Cooked baby clam meat | 1/2a | + | + | − |
A42 | 2001 | Mozzarella curd | 1/2a | + | + | − |
A67 | 2001 | Frozen capelin roe | 1/2a | + | + | − |
A74 | 2003 | Avocado puree for ice cream | 1/2a | + | + | − |
A75 | 2003 | Smoked salmon | 1/2a | + | + | − |
A94 | 2004 | Cheese chile relleno | 1/2a | + | + | − |
A96 | 2004 | Breaded catfish | 1/2a | + | + | − |
A97 | 2004 | Avocado puree | 1/2a | + | + | − |
AT-05 | 1987 | Raw milk | 1/2a | + | + | − |
AT-01 | 1986 | Brie cheese | 1/2a | + | + | − |
These isolates were resistant to Sau3AI, but their RM cassettes were not homologous to those of strain F2365; the isolates are designated “x-RM” in Fig. 4.
NT, not tested. Genome sequence analysis revealed the presence of the RM genes, as described in Results.
QC, quality control.