TABLE 2.
Primer name | Sequence (5′-3′)a |
---|---|
LMO0321RMdelF | GCATTCTAGACATCCGAATGTCTTCCTC |
LMO0324RMdelR | GCTCGGATCCCACTTCCAAAGTGGCAAATAA |
LMO0328RMdelF | CACTGGATCCAACATGGTATAGAGATTG |
LMO0329RMdelR | ATACGAATTCTTTTTCATCTGCTTTGACATC |
LMO0325R | GCTGAAGTTACTTCTGAAG |
0325F | CTGAGATTTTGAAGCACCGG |
0327R | CGTCGTCCATATTCTGCCGCAT |
Underlined portions of sequences indicate restriction sites used for ligations: TCTAGA, XbaI; GGATCC, BamHI; and GAATTC, EcoRI.