TABLE 1.
Bacterial strains used in this study
| Straina | Description | Reference or source |
|---|---|---|
| Ralstonia eutropha | ||
| H16 | Wild type | DSM 428 |
| ΔphaA | phaA precise-deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔbktB | bktB precise-deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔH16_A1528 | H16_A1528 precise-deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔH16_B1369 | H16_B1369 precise-deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔH16_A0170 | H16_A0170 precise-deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔH16_B0381 | H16_B0381 precise-deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔH16_A1713ΔH16_B1771 | H16_A1713/H16_B1771 precise-deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔphaAΔbktB (Δ2) | phaA and bktB deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔphaAΔbktBΔH16_A0170 (Δ3) | phaA, bktB, and H16_A0170 deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔphaAΔbktBΔH16_A1713ΔH16_B1771 (Δ4) | phaA, bktB, H16_A1713, and H16_B1771 deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔphaAΔbktBΔH16_A1713 ΔH16_B1771ΔH16_B1369 (Δ5) | phaA, bktB, H16_A1713, H16_B1771, and H16_B1369 deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔphaAΔbktBΔH16_A1713 ΔH16_B1771ΔH16_B1369ΔH16_A1528 (Δ6) | phaA, bktB, H16_A1713, H16_B1771, H16_B1369 and H16_A1528 deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔphaAΔbktBΔH16_A1713ΔH16_B1771 ΔH16_B1369ΔH16_A1528ΔH16_A0170 (Δ7) | phaA, bktB, H16_A1713, H16_B1771, H16_B1369, H16_A1528, and H16_A0170 deletion gene replacement strain derived from R. eutropha H16 | This study |
| ΔphaAΔbktBΔH16_A1713ΔH16_B1771 ΔH16_B1369ΔH16_A1528ΔH16_A0170Δ H16_B0381(Δ8) | phaA, bktB, H16_A1713, H16_B1771, H16_B1369, H16_A1528, H16_A0170, and H16_B0381 deletion gene replacement strain derived from R. eutropha H16 | This study |
| PHB−4 | Poly(3HB)-negative mutant | DSM 541 |
| Escherichia coli | ||
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) f80lacZ ΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| S17-1 | thi proA hsdR17 hasdM+ recA RP4 tra function | 40 |
a
The short-form designations of multiple mutants are shown in parentheses.