TABLE 3.
Homologue | Gene | Protein | e-valuea | No. of nucleotides/no. of amino acids | Mol wt | % identitya at: |
Gene expressionb | |
---|---|---|---|---|---|---|---|---|
Amino acid level | Nucleotide level | |||||||
1 | H16_A1438 (phaA) | Acetyl-CoA acetyltransferase | 0.0 | 1,182/393 | 40,548.8 | 100 | 100 | + |
2 | H16_A1445 (bktB) | β-Ketothiolase | 3e−95 | 1,185/394 | 40,903.8 | 52 | 65 | + |
3/4 | H16_A1713/H16_B1771 | Acetyl-CoA acetyltransferase | 8e−87 | 1,188/395 | 40,827.5 | 47 | 58 | + |
5 | H16_A0170 | Acetyl-CoA acetyltransferase | 1e−85 | 1,179/392 | 41,031.1 | 47 | 62 | + |
6 | H16_B0381 | Acetyl-CoA acetyltransferase | 9e−81 | 1,179/392 | 40,713.6 | 45 | 60 | − |
7 | H16_B1369 | Acetyl-CoA acetyltransferase | 3e−75 | 1,209/402 | 43,081.1 | 44 | 60 | − |
8 | H16_B0200 (pcaF) | β-Ketoadipyl CoA thiolase | 3e−71 | 1,203/400 | 41,889.0 | 44 | 59 | − |
9 | H16_A1528 | Acetyl-CoA acetyltransferase | 2e−53 | 1,179/392 | 41,057.1 | 39 | 57 | + |
10 | H16_A0462 | Acetyl-CoA acetyltransferase | 4e−55 | 1,197/398 | 41,776.1 | 38 | 58 | − |
11 | H16_B0668 | Acetyl-CoA acetyltransferase | 3e−55 | 1,203/400 | 41,992.9 | 40 | 56 | − |
12 | H16_A1720 | Acetyl-CoA acetyltransferase | 2e−52 | 1,182/393 | 41,500.4 | 41 | 55 | − |
13 | H16_B0759 | Acetyl-CoA acetyltransferase | 8e−52 | 1,176/391 | 40,515.5 | 38 | 56 | − |
14 | H16_B0662 | Acetyl-CoA acetyltransferase | 5e−50 | 1,152/383 | 40,436.2 | 37 | 54 | − |
15 | H16_A1887 | Acetyl-CoA acetyltransferase | 2e−60 | 1,179/392 | 41,525.3 | 37 | 54 | − |
The e-value (http://blast.ncbi.nlm.nih.gov/Blast.cgi) and amino acid and nucleotide acid identities are with respect to phaA.
Gene expression in R. eutropha H16 as taken from the raw data set obtained by Peplinski et al. (28). +, gene expression was detected; −, gene expression was not detected.