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SDS-PAGE (a) and Western blot analysis (b) of PhyC-R and PhyC-N preparations before and after N-deglycosylation by Endo Hf: Lanes M, protein molecular mass markers; lanes 1, PhyC-N before N-deglycosylation; lanes 2, PhyC-N after N-deglycosylation; lanes 3, PhyC-R before N-deglycosylation; lanes 4, PhyC-R after N-deglycosylation; lanes 5, Endo Hf.
