TABLE 1.
Bacterial strains and plasmids
| Bacterial strain or plasmid | Relevant characteristics or purposea | Reference(s) or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Multipurpose cloning | 69 |
| S17-1 | Conjugal transfer of mobilizable plasmids | 73 |
| P. syringae pv. syringae | ||
| B728a | Rifampin-resistant mutant of a wild-type strain originally collected from bean leaves | 45 |
| B728a ΔPsyr_3747 | Tetr | This study |
| B728a (pPnaggn:gfp) | Isolate B728a harboring NAGGN reporter plasmids containing transcriptional fusions of the promoter for Psyr_3747 with a GFP-encoding gene | This study |
| B728a (p519gfp) | Isolate B728a harboring constitutive p519gfp | 52 |
| P. syringae pv. tomato DC3000 | Rifampin derivative of DC52 (wild-type P. syringae pv. tomato race 0 strain), pathogen of tomato and Arabidopsis spp. | 20 |
| P. stutzeri DSM 5190T | Wild type, contains the full functional ectABC gene cluster | 67 |
| H. elongata | ||
| DSM 2581T | Wild type, contains the full functional ectABC gene cluster | 79 |
| WUB01 | Isolate DSM2581 ΔectC (in-frame deletion) | E. Witt and A. Ures, Galinski group, University of Bonn, Germany |
| WUB pPESyC | Isolate DSM2581 ΔectC plus ectC gene of P. syringae pv. syringae B728a; Chlr | This study |
| WUB pPEStC | Isolate DSM2581 ΔectC plus ectC gene of P. stutzeri DSM5190T; Chlr | This study |
| Plasmids | ||
| pENTR/D-Topo | Entry vector | Invitrogen |
| pLVC-D | Destination vector | 47 |
| pPE | Cloning vector; pBBR1MCS derivative equipped with the salt-dependent H. elongata promoter region upstream of H. elongata ectC | 12, 35, 36 |
| pPESyC | ectC gene of P. syringae pv. syringae B728a subcloned in frame into pPE under the control of the H. elongata promoter | This study |
| pPEStC | ectC gene of P. stutzeri DSM5190T subcloned in frame into pPE under the control of the H. elongata promoter | This study |
| pTOPO-Pnaggn | Vector equipped with the upstream promoter region of the NAGGN biosynthetic gene cluster | This study |
| pPROBE-OT | Vector containing a promoterless gfp gene | 54 |
| pPnaggn:gfp | Cloning vector containing the upstream promoter region of the NAGGN cluster fused with the gfp gene | This study |
a
Chlr, chloramphenicol resistant; Tetr, tetracycline resistant.