TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a |
|---|---|
| pp1 | 5′-(p)CATCTGCGACTGCAGG |
| pp2 | GGCATCGAGCTGTGCGAACTGCACAAGG |
| pp3 | CCTGCGTGCGCAGCTGGCCGACAC |
| pp4 | CGCACGCATGTCAGGGGTGCCTTCG |
| pp5 | CGCTCGTAGAGCTTGGACAGGCCCC |
| pp6 | 5′-(p)GCCCATCTGCGACTG |
| pp7 | CATTGTGAAACCGCTGGCGACTTCGG |
| pp8 | CGAGGAAGAGCACATCGACTGGCTGG |
| pp9 | GCCCAGGTCCTGAACGTTAGGAATACC |
| pp10 | CGTGCTTGCCGAGTTTGTTCAGCCC |
| pp11 | CGGAATTCCGAGCCGCGACGAGAACCCATTCGAC |
| pp12 | TGCTCTAGAGCAGGTCGCGTGCCGCCAGTTCGCCC |
| pp13 | TGCTCTAGAGCACCACAGACTCAAGTAAAAGCCCC |
| pp14 | CCCAAGCTTGGGCCTTTACCCCTTGGCCGCCACC |
| pp15 | CGGAATTCCGCTCATGACAGGCCGACTCCGTCG |
| pp16 | TGCTCTAGAGCACCAATCTTCGTACATGCGTGCG |
| pp17 | TGCTCTAGAGCACGCCCCATAAAAAAACCCGCTGATCG |
| pp18 | CCCAAGCTTGGGCGCGTACTGGCTCACTGGAGGTCG |
| pp19 | GCGGATCCGGCTTACGGCG |
| pp20 | CGGTGCTACCAACCTCCAGG |
| pp21 | TTCACATGGAGAATCAGGGCG |
| pp22 | CCTGGAGGTTGGTAGCACCG |
| pp23 | GCAATACCGTGGTGGTGATCG |
| pp24 | CGAGCCGCCACCAATGTCGCC |
| pp25 | GGGCTGAACAAACTCGGCAAGCACG |
| pp26 | ACGCTGCGCTCAATGGCGTACCC |
| pp27 | ATGAAAGGCGACGTAAGCG |
| pp28 | CTGCAAACCCTGATCAAGG |
a
Restriction sites on the primers are underlined. EcoRI was engineered on the 5′ ends of pp11 and pp15; XbaI was engineered on the 5′ ends of pp12 and pp13; HindIII was engineered on the 5′ ends of pp14 and pp18. 5′-(p), 5′ phosphorylated.