FIG. 3.

RT-PCR analysis of the transcriptional organization around the oxc gene in B. animalis subsp. lactis BI07. G, positive genomic DNA control; +, first-strand cDNA generated by RT reaction; −, negative-control reaction lacking RT enzyme. For cDNA synthesis, total RNA extracted from B. animalis subsp. lactis BI07 cells grown in a batch fermentor at pH 6.5 for 24 h was used as a template. B, F, G, I, J, N, O, and R, primers used for RT-PCR (see Table 1); M, molecular weight marker (M1, lambda DNA/EcoRI + HindIII; M2, lambda DNA/HindIII; M-Medical Fermentas, Milan, Italy). The expected size of amplicons is indicated on each side in bp.