FIG. 3.

PCR results with Cil1- and Cil2-specific primers. Shown are the results of three PCRs with a single cell of morphotype Cil1 (lanes 1, 2, and 3) or Cil2 (lanes 7, 8, and 9); reactions for the original (i.e., before culturing) samples 1 (lane 4) and 2 (lane 5) and reactions for the ambient water sample (lane 6) are also shown. Autoclaved distilled water was used as a negative control for each primer set (lane 10). Lanes M on the left, middle, and the right contain molecular size standards, with the molecular sizes of the bands indicated on the right. The molecular size of the PCR products, essentially the same as for the Cil1- and Cil2-specific primers, is marked on the right with an arrow.