TABLE 1.
Serotyping and phylogenetic characteristics of tested E. coli O156 isolates classified by the time pattern of isolationa
| Sampling day | Source | Isolate | Serotype by agglutination | Result by: |
Plasmid size(s) in kb | Cluster | |||
|---|---|---|---|---|---|---|---|---|---|
| MLST |
DNA-based serotyping |
||||||||
| ST | CC | MboII RFLP | fliC | ||||||
| 211 | Cow 26 (farm B) | WH-02/26/008-10 | O156:H25 | 688 | NA | O156 | H25 | 75, 6.9, and 5.1 | 8 |
| 274 | Cow 25 (farm B) | WH-02/25/010-9 | O156:Hnt | 688 | NA | O156 | H25 | 75, 6.9, and 5.1 | 8 |
| WH-02/25/010-10 | O156:H25 | 688 | NA | O156 | H25 | 75, 6.9, and 5.1 | 8 | ||
| 330 | Cow 16 (farm C) | WH-04/16/007-2 | O156:H− | 58 | 155 | ? | H25 | 155 | 1 |
| 365 | Cow 25 (farm C) | WH-04/25/005-1 | O156:H25 | 300 | NA | O156 | H25 | 75 | 8 |
| 400 | Cow 4 (farm B) | WH-02/04/017-3 | O156:Hnt | 300 | NA | O156 | H25 | 75 | 7 |
| WH-02/04/017-9 | O156:Hnt | 300 | NA | O156 | H25 | 75 | 7 | ||
| 512 | Cow 28 (farm B) | WH-02/28//018-1 | O156:H25 | 300 | NA | O156 | H25 | 75 | 6 |
| WH-02/28//018-3 | O156:H25 | 300 | NA | O156 | H25 | 75 | 6 | ||
| WH-02/28//018-4 | O156:H25 | 300 | NA | O156 | H25 | 75 | 6 | ||
| WH-02/28//018-6 | O156:H− | 300 | NA | O156 | H25 | 75 | 6 | ||
| 533 | Cow 25 (farm B) | WH-02/25/019-1 | O156:H25 | 300 | NA | O156 | H25 | 75 | 7 |
| WH-02/25/019-9 | O156:H25 | 300 | NA | O156 | H25 | 75 | 7 | ||
| 547 | Cow 12 (farm D, group 1) | WH-03/12/016-1 | O156:H− | 300 | NA | O156 | H25 | 75 and 6.9 | 7 |
| WH-03/12/016-2 | O156:H− | 300 | NA | O156 | H25 | 75 and 6.9 | 7 | ||
| WH-03/12/016-8 | O156:Hnt | 300 | NA | O156 | H25 | 75 and 6.9 | 7 | ||
| 554 | Cow 23 (farm B) | WH-02/23/021-2 | O156:Hnt | 300 | NA | O156 | H25 | 75 | 7 |
| WH-02/23/021-3 | O156:Hnt | 300 | NA | O156 | H25 | 75 | 7 | ||
| WH-02/23/021-5 | O156:Hnt | 300 | NA | O156 | H25 | 75 | 7 | ||
| WH-02/23/021-9 | O156:H25 | 300 | NA | O156 | H25 | 75 | 7 | ||
| WH-02/23/021-10 | O156:H25 | 300 | NA | O156 | H25 | 75 | 7 | ||
| 638 | Cow 25 (farm B) | WH-02/25/024-3 | O156:H− | 300 | NA | O156 | H25 | 75 | 7 |
| WH-02/25/024-5 | O156:H25 | 300 | NA | O156 | H25 | 75 | 7 | ||
| 708 | Cow 2 (farm D, group 2) | WH-05/02/001-2 | O156:H25 | 1308 | NA | ? | H46 | 110, 85, and 5.1 | 2 |
| WH-05/02/001-3 | O156:Hnt | 300 | NA | O156 | H25 | 75 and 6.9 | 7 | ||
| WH-05/02/001-8 | O156:H25 | 1308 | NA | ? | H46 | 110, 85, and 5.1 | 2 | ||
| WH-05/02/001-9 | O156:Hnt | 300 | NA | O156 | H25 | 75 and 6.9 | 7 | ||
| 764 | Cow 23 (farm D, group 2) | WH-05/23/003-1 | O156:H25 | 10 | 10 | ? | H11 | 155, 110, 75, 50, 21, 5.9, and 3.1 | 3 |
| Cow 25 (farm D, group 2) | WH-05/25/003-1 | O156:H25 | 48 | 10 | O103 | H43 | 75, 35, 21, 7.5, and 2.9 | 4 | |
| 792 | Cow 25 (farm D, group 2) | WH-05/25/004-1 | O156:H25 | 300 | NA | O156 | H25 | 75 and 6.9 | 5 |
| WH-05/25/004-3 | O156:H25 | 10 | 10 | ? | H11 | 155, 110, 75, 50, 21, 5.9, 3.1, and 2.9 | 3 | ||
| 841 | Cow 12 (farm D, group 2) | WH-05/12/006-2 | O156:Hnt | 327 | NA | ? | H8 | 85 | 4 |
a
CC, clonal complex; MLST, multilocus sequence typing; ST, sequence type; NA, CC not assigned; H−, nonmotile; Hnt, not typeable; ?, not detectable.