TABLE 2.
Quantitation of viral proteins incorporated into influenza A virus particles expressing M2 proteins with an S71Y mutation
| Figure | Protein | Virus | Amount of protein in MDCK cellsa |
|---|---|---|---|
| 7C | Total NP | rUdorn | 0.20 ± 0.04 |
| rUdorn-M2Y76A | 0.10 ± 0.02* | ||
| rUdorn-M2S71Y | 0.16 ± 0.03* | ||
| rUdorn-M2S71Y/Y76A | 0.15 ± 0.02 | ||
| NPa | rUdorn | 0.16 ± 0.04 | |
| rUdorn-M2Y76A | 0.044 ± 0.008* | ||
| rUdorn-M2S71Y | 0.14 ± 0.03 | ||
| rUdorn-M2S71Y/Y76A | 0.12 ± 0.02 | ||
| M1 | rUdorn | 1.5 ± 0.4 | |
| rUdorn-M2Y76A | 0.8 ± 0.2 | ||
| rUdorn-M2S71Y | 1.4 ± 0.3 | ||
| rUdorn-M2S71Y/Y76A | 1.2 ± 0.3 | ||
| Total M2 | rUdorn | 0.030 ± 0.005 | |
| rUdorn-M2Y76A | 0.018 ± 0.002 | ||
| rUdorn-M2S71Y | 0.021 ± 0.006 | ||
| rUdorn-M2S71Y/Y76A | 0.017 ± 0.004 | ||
| M2a | rUdorn | 0.014 ± 0.003 | |
| rUdorn-M2Y76A | 0.007 ± 0.001 | ||
| rUdorn-M2S71Y | 0.017 ± 0.006 | ||
| rUdorn-M2S71Y/Y76A | 0.008 ± 0.002 |
a
Virus proteins were quantified, and amounts are expressed relative to the amount of HA protein. Three or four independent experiments were averaged and the standard error is shown. M2a, full-length M2; *, P ≤ 0.05.