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. 2010 Jun 10;84(17):8673–8682. doi: 10.1128/JVI.00641-10

FIG. 1.

FIG. 1.

Transduction and circularization with ssAAV and scAAV in ATM−/− cells. (a) Normal or ATM−/− transformed fibroblasts were infected with four different viral vectors containing either an intact GFP gene (ssAAV-GFP and scAAV-GFP) or a circularization-dependent GFP gene (ssAAV-GFP-CD and scAAV-GFP-CD) at an MOI of 1 for 2 h. GFP expression was quantified by flow cytometry at 24 h postinfection. The indicated (* and **) differences between wt and ATM−/− cells were significant (P < 0.0001). (b) Drug inhibition of ATM kinase activity. Transformed wt fibroblasts were treated with 10 μM KU 55933, a selective inhibitor of ATM kinase activity, for 24 h before infection with the four reporter vectors. Dimethyl sulfoxide (DMSO) was added in negative-control cells at the same final concentration. Transduction was assayed at 24 h postinfection. The indicated (* and **) differences were significant (P < 0.0001); P = 0.0768 for ssAAV-CD, and P = 0.0001 for scAAV-CD. Error bars indicate standard deviations.