FIG. 7.

Integration assay for ssAAV-GFP and scAAV-GFP vectors in ATM^−/− cells. Cell lines (HeLa, wt fibroblast, and ATM ^−/− fibroblast) were infected at an MOI of 1,400 for 2 h and passed every 4 to 7 days. Samples were taken at passage on the indicated days postinfection and fixed for quantification of GFP expression by flow cytometry. Infected cultures were passed until a stable percentage of cells expressed GFP, indicating that the vector was chromosomally integrated. Error bars indicate standard deviations.