FIG. 1.

Myc knockdown induces KSHV reactivation. (A) BC-3-G cells that were mock transduced or transduced with the indicated shRNA vectors were imaged with fluorescence microscopy for enhanced green fluorescence protein (EGFP) expression at day 3 posttransduction. Magnification, ×200. (B) BC-3 or BCBL-1 cells were mock transduced or transduced with the indicated shRNA vectors. At day 3 posttransduction, whole-cell lysates were collected and analyzed for KSHV K8 and/or Myc expression by Western blotting. An actin immunoblot is shown as a loading control. (C) BC-3 cells were cotransfected with the indicated shRNA plasmids. At day 3 posttransduction, whole-cell lysates were collected and analyzed for KSHV RTA expression by Western blotting. A tubulin immunoblot is shown as a loading control. Data for all panels are representative of three independent experiments.