FIG. 2.

Myc represses RTA transcription. (A) BC-3 cells were transduced with the indicated shRNA vectors. At day 2 posttransduction, the relative RTA mRNA levels were measured by reverse transcription-quantitative PCR (RT-Q-PCR), normalized to the actin mRNA levels, and displayed as fold induction over the shCtrl control. (B) 293T cells were cotransfected with pKRp (RTA promoter luciferase reporter plasmid) or pGL2 (control reporter plasmid) plus the indicated shRNA plasmids. At 48 h posttransfection, the luciferase activities were measured and are presented as relative fold induction over the luciferase activity of the pGL2-plus-shCtrl sample. (C) 293T cells were cotransfected with the indicated luciferase reporter and expression plasmids. At 48 h posttransfection, the luciferase activities were determined and are presented as relative fold induction over the luciferase activity of the pGL2-plus-Ctrl sample. (D) 293T cells were cotransfected with pPAN-Luc (PAN promoter luciferase reporter plasmid) and the indicated expression plasmids. At 48 h posttransfection, the luciferase activities were determined and are presented as relative fold induction over the luciferase activity of the sample without Myc and RTA. Data are the means ± standard deviations from three independent experiments.