FIG. 4.
Myc knockdown induces G1 cell cycle arrest and apoptosis. (A) BC-3 cells were transduced with the shCtrl or shMyc-1 lentiviral vector. Cell numbers were determined by the trypan blue exclusion assay at day 4 posttransduction and are displayed as relative cell densities normalized to the number of shCtrl-transduced cells. Data are the means ± standard deviations from three independent experiments. (B) Flow cytometry evaluation of the cell cycle in shCtrl- and shMyc-1-transduced BC-3 cells at day 4 posttransduction. The percentage of cells in each phase of the cell cycle was determined by analysis performed with Modfit LT software. Data are representative of two independent experiments.