FIG. 1.
T- and B-lymphocyte cell lines support adenovirus productive infection. Two human T-cell lines, Jurkat and KE37(DSMZ), and two human B-cell lines, BJAB and Ramos, were infected with adenovirus Ad5dl309 at 100 PFU/cell. (A) Viral late gene expression was monitored for 10 days postinfection by intracellular staining for the hexon protein using antihexon antibody from Chemicon (MAB8051) and a secondary antibody, R-PE-goat F(ab′)2 anti-mouse IgG from Southern Biotechnologies. (B) Viral DNA replication was quantified by real-time PCR. (C to F) Growth and viability of infected cells (closed symbols) and uninfected cells (open symbols) were monitored over the 10-day period. The number of viable cells was determined daily. Cells were subcultured at 2.5 × 105 viable cells/ml on the indicated day. Lines represent a best-fit exponential regression.