FIG. 11.

Restoration of cell surface CAR levels in persistently infected BJAB cells does not render the cells able to support a productive infection. BJAB cells were infected with Ad5dl309 and maintained as a persistently infected culture for 190 days before being transduced with an empty retrovirus vector (LXSN) or a CAR-expressing retrovirus vector. The transduced BJAB cells were maintained under selection for the integrated retrovirus for an additional 40 days before being reinfected with Ad5dl309 at 100 PFU/cell. (A) The fraction of CAR-positive cells at the time of reinfection was determined by flow cytometry. (B) Reinfected cells were harvested at 3, 6, and 10 days after reinfection, and the level of viral DNA was quantified by real-time PCR. (C) Viral late protein expression was measured at the times indicated by intracellular staining for hexon and flow cytometry.