FIG. 4.

Southern blot analysis of adenovirus DNA in persistently infected BJAB cells. BJAB cells were infected with wild-type adenovirus Ad2 for 234 days or Ad5dl309 for 180 days before total infected cell DNA was extracted. (A) For Ad5dl309-infected BJAB cells, 500 ng of purified Ad5dl309 or 10 μg of total cellular DNA was digested with restriction enzymes FseI or RsrII, separated by electrophoresis, and hybridized with ³²P-radiolabeled Ad5dl309 DNA. (B) For Ad2-infected BJAB cells, purified Ad2 DNA or 10 μg of total cellular DNA was cleaved with BamHI or SpeI before analysis. The predicted restriction fragments are indicated in kbp above each blot, and at the approximate position is indicated along the blots. Terminal fragments are indicated by symbols with the closed circle.