FIG. 6.

Loss of cell surface CAR protein requires late events in the virus life cycle. (A) BJAB cells were infected with replication-defective AdCMV-GFP or replication-competent Ad007EGFP virus. The fraction of cells containing CAR on their surfaces was determined by flow cytometry. The fraction of cells that were productively infected was measured by GFP fluorescence using flow cytometry at the indicated times postinfection. (B) BJAB cells were infected with adenovirus 12S-WT or H5wt300-ΔpTP and harvested at 3, 6, or 10 days postinfection. Viral DNA replication was quantified by real-time PCR. (C) The infected BJAB cells analyzed in panel B were evaluated for hexon expression by intracellular staining and cell surface CAR expression with the RmcB antibody using flow cytometry.