FIG. 11.

(a) Induction of ISG mRNA by rIFN-γ in TO cells. Purified rIFN-γ was serially diluted from 0.33 mg/ml in cell medium and incubated with TO cells for 24 h. The data are expressed as the mean fold changes in gene expression ± standard errors of different dilutions of the IFN-γ-treated group relative to the nontreated control group after normalization to β-actin (n = 2) (±SEM). (b) Cytopathic effect reduction assay of IFN-γ-treated TO cells infected with SAV-3. TO cells were stimulated with serial dilutions of rIFN-γ and trIFN-γ for 24 h and subsequently infected with 1 MOI of SAV-3. There is no significant difference in protection against CPE between cell cultures pretreated with rIFN-γ and trIFN-γ (n = 4) (±SEM). Non-tr, nontreated cells; Non-inf, noninfected; OD490, optical density at 490 nm. (c) Real-time PCR quantification of virus in cell supernatants from TO cells stimulated with serial dilutions of mature IFN-γ for 24 h and subsequently infected with 1 MOI of SAV-3. Cell supernatants were collected when a strong CPE was observed in the control infected cells (8 days postinfection). The data are expressed as the mean crossing-point values ± standard errors using real-time PCR of different dilutions of an IFN-γ-treated group relative to the nontreated infected control group (n = 2) (±SEM).