FIG. 8.

Timing of IFN-α treatment of SAV-3-infected cells. Treatment 4 to 24 h prior to infection results in a marked reduction in virus replication (2,000- to 40,000-fold reduction) as measured by real-time PCR and protection against CPE. rIFN-α treatment at the time of infection or up to 24 h postinfection gives a reduction in virus replication but not protection against CPE. TO cells were infected with an MOI of 1, and collection was done at 4 days postinfection. Shown are representative data from two independent experiments, expressed relative to the nontreated control (NT). More details are given in Materials and Methods.