TABLE 1.
Primer and probe sequences for cloning and quantitative real-time PCRa
| Primer | Sequence | Use | GenBank accession no. |
|---|---|---|---|
| IFNα-F1 | CAGTATGCAGAGCGTGTGT | pGEMT cloning | AY216594 |
| IFNα-R1 | CGTAGCTTCTGAAATGAGTCTGG | ||
| pET-IFNα-F1 | GCGCATATGTGTGACTGGATCCGACAC | pET32 cloning | |
| pET-IFNα-R1 | GCGCTCGAGGTACATCTGTGCTGCAAG | ||
| pET-IFNα-R2 | GCGCTCGAGGTTCATTTTTCTCAGAAC | ||
| IFNα-F2 | TGGGAGGAGATATCACAAAGC | qPCR | |
| IFNα-R2 | TCCCAGGTGACAGATTTCAT | ||
| Mx-F | TGCAACCACAGAGGCTTTGAA | qPCR | U66475 |
| Mx-R | GGCTTGGTCAGGATGCCTAAT | ||
| pET14b-E2-F | GCGCATATGGCTGTGTCTGCGTCGCCT | pET14b cloning | |
| pET14b-E2-R | GCGCTCGAGTTACGCACGAGCCCCAGG | ||
| SAV-3 E2-F | CAGTGAAATTCGATAAGAAGTGCAA | qPCR | EF675594 |
| SAV-3 E2-R | TGGGAGTCGCTGGTAAAGGT | ||
| E2 probe | FAM-5′-AGCGCTGCCCAAGCGACCG-3′-MGB | ||
| IFNγ-F1 | AGGCGGTCTCGTTAAGTCAA | pGEMT cloning | AY795563 |
| IFNγ-R1 | TAAACTGACCCAAGATCAGC | ||
| pET32-IFNγ-F | GCGCATATGGCTCAGTACACATCAATT | pET32 cloning | |
| pET32-IFNγ-R1 | GCGCTCGAGCATGATGCTTGATTTGAG | ||
| pET32-IFNγ-R2 | GCGCTCGAGTTTCTCGTAGATGGTAAT | ||
| IFNγ-F4 | CTAAAGAAGGACAACCGCAG | qPCR | |
| IFNγ-R4 | CACCGTTAGAGGGAGAAATG | ||
| γIP10-F | TGCCAGAACATGGAGATCAT | qPCR | EF619047 |
| γIP10-R | TTTACTGCACACTCCTTTGGTT | ||
| ISG15-F | AAGTGATGGTGCTGATTACGG | qPCR | AY926456 |
| ISG15-R | CACCGTTAGAGGGAGAAATG | ||
| β-actin-F | CCAGTCCTGCTCACTGAGGC | qPCR | AF012125 |
| β-actin-R | GGTCTCAAACATGATCTGGGTCA |
a
Restriction sites are underlined. qPCR, quantitative PCR; FAM, 6-carboxyfluorescein; MGB, MGB-TaqMan probe.