FIG. 1.

Induction of IFN and ISGs. A549 cells were infected with TBEV strains Neudörfl, Hypr, and Absettarov at an MOI 1, and total cell RNA was extracted at 24 h p.i. Levels of IFN-β, ISG15, and ISG56 mRNAs were measured by real-time RT-PCR analysis, normalized to the cellular γ-actin mRNA, and set in relation to mRNA levels of mock-infected cells. Mean values and standard deviations from three independent experiments are shown.