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. 2010 Jun 16;84(17):8980–8985. doi: 10.1128/JVI.00858-10

FIG. 2.

FIG. 2.

Inhibition of viral replication by feline TRIMCyp and its reversal by CypA antagonists. CrFK (ID10) cells stably transduced with a retroviral vector bearing feline TRIMCyp (B and D) or vector only (A and C) were infected with FIV-Fca (Petaluma-F14 strain) (A and B) or FIV-Pco (CoLV strain) (C and D). Infections were performed in the presence or absence of medium supplemented with 2.5 μM CypA antagonist NIM811 or Debio-025 or their respective solvents, DMSO and ethanol (EtOH). Supernatants were collected and assayed for RT activity by nonisotopic RT assay (LentiRT; CavidTech, Sweden). CON, control. (E to H) Syncytium formation in CrFK cells infected with FIV-Fca. Cells expressing vector only (E and G) or TRIMCyp (F and H) and infected with FIV-Fca in the presence of Debio-025 (G and H) or ethanol solvent control (E and F) were fixed and stained at 10 days postinfection with 1.0% methylene blue-0.2% basic fuchsin in methanol. The arrows indicate small syncytia, magnified in the inset (H).